Shanghai Institute of Precision Medicine

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WU Jian

Professor, co-Principal Investigator

Research direction:Structure and Function of Long non-coding RNA in Complex with Proteins

E-mail:wujian@@shsmu.edu.cn

Lab: http://www.shipm.cn/jyjz_web/html/jyjz_English/jyjz_eg_wj_sysjj/List/index.htm

Research Interests

  • Most of the eukaryotic genome is transcribed, yielding a complex network of transcripts that includes tens of thousands of long noncoding RNAs (lncRNAs) with little or no protein-coding capacity. Functioning in the form of ribonucleoprotein (RNP) complex, lncRNAs play significant roles in most biological processes such as stem cell pluripotency maintenance, cell differentiation, immunological regulation, carcinogenesis, et al. With a combination of structural biology, biochemistry, cell biology and genetics, We will focus on the structural basis of the lncRNA-protein complex assembly, the specificities of the protein-protein and protein-nucleic acid interactions, the molecular mechanism of enzymatic reaction and dynamic regulation. In addition, we will also study the relationship between dysfunctional telomeres and diseases, which will provide a solid foundation for our understanding of lncRNA-protein complex in maintaining chromosomal and genomic homeostasis and will open a new horizon on novel drug development.

Education and Work Experience

Education

  • 2003/09 – 2009/10, Shanghai Institute of Biochemistry and Cell Biology, SIBS, CAS

    Ph.D., Biochemistry and Molecular Biology

  • 1999/09 – 2003/06, Shandong University, College of Life Science

    B.S., Biology

Work Experience

  • 2018/01 – Present, Shanghai Jiao Tong University School of Medicine, Shanghai Ninth People’s Hospital

    Professor

  • 2017/11 – 2017/12, Shanghai Jiao Tong University School of Medicine, Shanghai Ninth People’s Hospital

    Associate Professor

  • 2014/11 – 2017/11, Shanghai Institute of Biochemistry and Cell Biology, SIBS, CAS

    Associate Professor

  • 2012/11 – 2014/11, Shanghai Institute of Biochemistry and Cell Biology, SIBS, CAS

    Assistant Professor

  • 2010/10 – 2012/04, University of Michigan, Department of Biochemistry

    Postdoc

  • 2009/10 – 2010/10, Shanghai Institute of Biochemistry and Cell Biology, SIBS, CAS

    Research Assistant

Selected Publications

  1. Chen H#, Xue J#, Churikov D, Hass E, Shi S, Lemon L, Luciano P, Bertuch A, Zappulla D, Geli V, Wu J*, and Lei M* (2018) Structural insights into yeast telomerase recruitment to telomere. Cell, 172(1-2), 331-343.

  2. Long, J#, Huang C#, Chen Y, Zhang Y, Shi S, Wu L, Liu Y, Liu C, Wu J*, and Lei M* (2017) Telomeric TERB1-TRF1 interaction is crucial for male meiosis. Nat Struct Mol Biol, 24(12), 1073-1080.

  3. Xue J#, Chen H#, Wu J, Takeuchi M, Inoue H, Liu Y, Chen Y*, Kanoh J*, and Lei M* (2017) Strcture of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex. Cell Res, 27(12), 1503-1520.

  4. Tan R#, Nakajima S, Wang Q, Sun H, Xue J, Wu J, Hellwig S, Zeng X, Yates NA, Smithgall TE, Lei M, Jiang Y, Levine AS, Su B*, and Lan L* (2017) Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1. Mol Cell, 66(5), 818-831.

  5. Liu X#, Wei W, Liu Y, Yang X, Wu J, Zhang Y, Zhang Q, Shi T, Du JX, Zhao Y, Lei M, Zhou JQ, Li J, and Wong J* (2017) MOF as an evolutionarily conserved histone crotonyltransferase and transcriptional activation by histone acetyltransferase-deficient and crotonyltransferase-competent CBP/p300. Cell Discov, celldisc.2017.16. eCollection 2017.

  6. Chen C#, Gu P#, Wu J, Chen X, Niu S, Sun H, Wu L, Li N, Peng J, Shi S, Fan C, Huang M, Wong CCL, Gong Q, Kumar-Sinha C, Zhang R, Pusztai L, Rai R, Chang S*, and Lei M* (2017) Structural insights into POT1-TPP1 interaction and POT1 C-terminal mutations in human cancer. Nat Commun, 8:14929.

  7. Li Y#, Han J, Zhang Y, Cao F, Liu Z, Li S, Wu J, Hu C, Wang Y, Shuai J, Chen J, Cao L, Li D, Shi P, Tian C, Zhang J, Dou Y, Li G*, Chen Y*, and Lei M* (2016) Structural basis for activity regulation of MLL family methyltransferases. Nature, 530, 447-452.

  8. Yin J#, Wan B#, Sarkar J#, Horvath K, Wu J, Chen Y, Cheng G, Wan K, Chin P, Lei M*, and Liu Y* (2016) Dimerization of SLX4 contributes to functioning of the SLX4-nuclease complex. Nucleic Acids Res, 44, 4871-4880.

  9. Wan B#, Tang T#, Upton H, Shuai J, Zhou Y, Li S, Chen J, Brunzelle J, Zeng Z, Collins K, Wu J*, and Lei M* (2015) The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex. Nat Struct Mol Biol, 22, 1023-1026.

  10. Deng W#, Wu J#, Wang F#, Kanoh J, Dehe P. Inoue H, Chen J, and Lei M* (2015) Fission yeast telomere-binding protein Taz1 is a functional but not a structural counterpart of human TRF1 and TRF2. Cell Res, 25, 881-884.

  11. Zhang Z#, Wu J, Lin W, Wang J, Yan H, Zhao W, Ma J, Ding J*, Zhang P*, and Zhao GP* (2014) Subdomain II of α-isopropylmalate synthase is essential for activity: inferring a mechanism of feedback inhibition. J Biol Chem, 289, 27966-27978.

  12. Huang J#, Brown A, Wu J, Xue J, Bley C, Rand D, Wu L, Zhang R, Chen J*, and Lei M* (2014) Structural Basis for Protein-RNA Recognition in Telomerase. Nat Struct Mol Biol, 21(6), 507-512.

  13. Wan B#, Yin J#, Horvath K#, Sarkar J, Chen Y, Wu J, Wan K, Lu J, Gu P, Yu EY, Lue NF, Chang S, Liu Y*, and Lei M* (2013) SLX4 assembles a telomere maintenance toolkit by bridging multiple endonucleases with telomeres. Cell Rep, 4(5), 861-869.