Research Interests

• Our lab aims to develop accurate and efficient nuclear and mitochondrial gene editing tools and to build genetic disease models by using CRISPR, TALEN and other systems. By using our developed gene editing tools, such as base editors and prime editors, in combination with classical epigenetic means and high-throughput sequencing technologies, the functional and regulatory mechanisms of epigenetic modifications and their associated factors in reproductive development, stem cell fate determination and human diseases are investigated in a high-throughput strategy.

Education and Work Experience

• 2022.9-present: Principal Investigator, Shanghai Institute of Precision Medicine, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine

• 2017.8-2022.8: Principal Investigator, Guangzhou University

• 2016.8-2017.8: Research Assistant Professor, iHuman Institute, ShanghaiTech University

• 2016.5-2016.7: Research Fellow, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences

• 2012.8-2016.4: Postdoc, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences

• 2006.9-2012.6: Ph.D, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences

• 2002.9-2006.6: B.S., Wuhan University

Selected Publications

1. Li, J.*, Lin, J.*, Huang, S.*, Li, M., Yu, W., Zhao, Y., Guo, J., Zhang, P., Huang, X.#, and Qiao, Y.# (2022) Functional Phosphoproteomics in Cancer Chemoresistance Using CRISPR-Mediated Base Editors. Adv Sci, e2200717

2. Li, J.*, Yu, W.*, Huang, S.*, Wu, S., Li, L., Zhou, J., Cao, Y., Huang, X.#, and Qiao, Y.# (2021) Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity. Nat Commun 12, 2287

3. Ye, B.*, Fan, D.*, Xiong, W.*, Li, M., Yuan, J., Jiang, Q., Zhao, Y., Lin, J., Liu, J., Lv, Y., Wang, X., Li, Z.#, Su, J.#, and Qiao, Y.# (2021) Oncogenic enhancers drive esophageal squamous cell carcinogenesis and metastasis. Nat Commun 12, 4457

4. Qiao, Y. *,#, Ren, C.*, Huang, S.*, Yuan, J.*, Liu, X., Fan, J., Lin, J., Wu, S., Chen, Q., Bo, X., Li, X., Huang, X., Liu, Z.#, and Shu, W.# (2020) High-resolution annotation of the mouse preimplantation embryo transcriptome using long-read sequencing. Nat Commun 11, 2653

5. Qiao, Y.*,#, Wang, Z.*, Tan, F.*, Chen, J.*, Lin, J., Yang, J., Li, H., Wang, X., Sali, A., Zhang, L.#, and Zhong, G.# (2020) Enhancer Reprogramming within Pre-existing Topologically Associated Domains Promotes TGF-beta-Induced EMT and Cancer Metastasis. Mol Ther 28, 2083-2095

6. Liu, J.*, Li, J.*, Zhou, C.*, Meng, B.*, Wei, Y.*, Yang, G., Lu, Z., Shen, Q., Zhang, Y., Yang, H.#, Qiao, Y. # (2019) Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9). Science Bulletin 64, 1592-1600

7. Yang, G.*, Zhou, C.*, Wang, R.*, Huang, S., Wei, Y., Yang, X., Liu, Y., Li, J., Lu, Z., Ying, W., Li, X., Jing, N., Huang, X.#, Yang, H.#, and Qiao, Y.# (2019) Base-Editing-Mediated R17H Substitution in Histone H3 Reveals Methylation-Dependent Regulation of Yap Signaling and Early Mouse Embryo Development. Cell Rep 26, 302-312