School of Pediatrics


Wu Zhixiang

Associate Professor


Tel. (0)86-25078413

Research Interests: Proteomics and precision medicine of pediatric solid tumor


Dr. Wu (b. 1981) received his PhD in 2012 from Technical University of Munich, where he was trained in tumor proteomics and molecular biology. Then, he joined Shanghai Institute of Materia Medica, Chinese Academy of Sciences as a post-doctor for chemical proteomics in drug discovery. Since 2014, he completed residency training and a fellowship in pediatric surgery at Xinhua hospital, School of Medicine, Shanghai Jiao Tong University. Now he is an Associate Professor of Department of Pediatric Surgery, Xinhua hospital and vice director for research in the Department of Pediatric Oncology, Shanghai Institute for Pediatric Research.


  1. Zhuang Y, Wu Z, Shi J, Wu Y, Lv F: Yolk sac tumor of biliary tract: A rare biliary space-occupying entity in children. Journal of Pediatric Surgery Case Reports 2022, 76:102102.

  2. Liu X, Cheng C, Cai Y, Gu Y, Wu Y, Chen K, Wu Z: Pan‑cancer analyses reveal the regulation and clinical outcome association of PCLAF in human tumors. International journal of oncology 2022, 60(6):1-13.

  3. Liu X, Cai Y, Cheng C, Gu Y, Hu X, Chen K, Wu Y, Wu Z: PCLAF promotes neuroblastoma G1/S cell cycle progression via the E2F1/PTTG1 axis. Cell death & disease 2022, 13(2):1-12.

  4. Pan L-J, Chen J-L, Wu Z-X, Wu Y-M: Exportin-t: A novel prognostic predictor and potential therapeutic target for neuroblastoma. Technology in Cancer Research & Treatment 2021, 20:15330338211039132.

  5. Pan L, Cheng C, Duan P, Chen K, Wu Y, Wu Z: XPO1/CRM1 is a promising prognostic indicator for neuroblastoma and represented a therapeutic target by selective inhibitor verdinexor. Journal of Experimental & Clinical Cancer Research 2021, 40(1):1-16.

  6. Liu X, Cheng C, Chen K, Wu Y, Wu Z: Recent progress in lymphangioma. Frontiers in Pediatrics 2021, 9.

  7. Hu X, Zhou Y, Hill C, Chen K, Wu Y, Li Z, Cheng C, Liu X, Duan P, Ewing R: Identification of MYCN non-amplified neuroblastoma subgroups points towards molecular signatures for precision prognosis and therapy stratification. 2021.

  8. Gu Y, Lv F, Wu Y, Wu Z: Angiomatoid fibrous histiocytoma with Castleman disease: A case report. Pediatric Blood & Cancer 2021, 68(2):e28590.

  9. Gu Y, Hu X, Liu X, Cheng C, Chen K, Wu Y, Wu Z: MCM6 indicates adverse tumor features and poor outcomes and promotes G1/S cell cycle progression in neuroblastoma. BMC cancer 2021, 21(1):1-14.

  10. Duan P, Huang Y, Chen K, Cheng C, Wu Z, Wu Y: 15, 16-dihydrotanshinone I inhibits EOMA cells proliferation by interfering in posttranscriptional processing of hypoxia-inducible factor 1. International Journal of Medical Sciences 2021, 18(14):3214.

  11. Cheng C, Cai Y, Liu X, Wu Y, Wu Y, Wu Z: KHSRP Modulated Cell Proliferation and Cell Cycle Via Regulating PPP2ca and p27 Expression in Wilms Tumor. Available at SSRN 4001781 2021.

  12. Cai Y, Wu Y, Wu Z, Liu X, Pan W: Comparative Study of Thoracoscopic and Modified Small Incision Repair for Congenital Diaphragmatic Eventration in Children. Journal of Laparoendoscopic & Advanced Surgical Techniques 2021, 31(9):1079-1083.

  13. Xu J-Y, Zhang C, Wang X, Zhai L, Ma Y, Mao Y, Qian K, Sun C, Liu Z, Jiang S: Integrative proteomic characterization of human lung adenocarcinoma. Cell 2020, 182(1):245-261. e217.

  14. Pan L, Nie L, Yao S, Bi A, Ye Y, Wu Y, Tan Z, Wu Z: Bufalin exerts antitumor effects in neuroblastoma via the induction of reactive oxygen species‑mediated apoptosis by targeting the electron transport chain. International journal of molecular medicine 2020, 46(6):2137-2149.

  15. Chen K, Cai Y, Cheng C, Zhang J, Lv F, Xu G, Duan P, Wu Y, Wu Z: MYT1 attenuates neuroblastoma cell differentiation by interacting with the LSD1/CoREST complex. Oncogene 2020, 39(21):4212-4226.

  16. Cai Y, Chen K, Cheng C, Xu Y, Cheng Q, Xu G, Wu Y, Wu Z: Prp19 is an independent prognostic marker and promotes neuroblastoma metastasis by regulating the Hippo-YAP signaling pathway. Frontiers in oncology 2020, 10:575366.

  17. Cheng C, Wu Y, Pan W, Wang J, Wu Z, Lv F: Follow-up report of laparoscopic fundoplication in different types of esophageal hiatal hernia in children. Journal of Laparoendoscopic & Advanced Surgical Techniques 2019, 29(10):1320-1324.

  18. Chen H, Lin H, Xu M, Xu G, Fang X, He L, Chen Z, Wu Z, Geng H: Quantitative urinary proteome reveals potential biomarkers for ureteropelvic junction obstruction. PROTEOMICS–Clinical Applications 2019, 13(4):1800101.

  19. Zhang M, Jiang Z, Chen S, Wu Z, Chen K, Wu Y: Legumain correlates with neuroblastoma differentiation and can be used in prodrug design. Chemical Biology & Drug Design 2018, 91(2):534-544.

  20. Gu Y, Lv F, Xue M, Chen K, Cheng C, Ding X, Jin M, Xu G, Zhang Y, Wu Z: The deubiquitinating enzyme UCHL1 is a favorable prognostic marker in neuroblastoma as it promotes neuronal differentiation. Journal of Experimental & Clinical Cancer Research 2018, 37(1):1-14.

  21. Chen K, Cai Y, Zhang M, Wu Z, Wu Y: Both serum and tissue galectin‐1 levels are associated with adverse clinical features in neuroblastoma. Pediatric blood & cancer 2018, 65(9):e27229.

  22. Cai Y, Lv F, Kaldybayeva N, Zhamilya A, Wu Z, Wu Y: 15, 16-Dihydrotanshinone I inhibits hemangiomas through inducing pro-apoptotic and anti-angiogenic mechanisms in vitro and in vivo. Frontiers in Pharmacology 2018, 9:25.

  23. Wenbo Yan YW, Zhixiang Wu, Yiming Gong, Chengren Shi, Wang J: Gastroschisis in monochorionic male twins. Pediatric Surgery International 2017, 33:627–629.

  24. Wang Y, Chen K, Cai Y, Cai Y, Yuan X, Wang L, Wu Z, Wu Y: Annexin A2 could enhance multidrug resistance by regulating NF-κB signaling pathway in pediatric neuroblastoma. Journal of Experimental & Clinical Cancer Research 2017, 36(1):1-16.

  25. Nagel M-K, Kalinowska K, Vogel K, Reynolds GD, Wu Z, Anzenberger F, Ichikawa M, Tsutsumi C, Sato MH, Kuster B: Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proceedings of the National Academy of Sciences 2017, 114(34):E7197-E7204.

  26. Liu B, Lu H, Li D, Xiong X, Gao L, Wu Z, Lu Y: Aberrant expression of FBXO2 disrupts glucose homeostasis through ubiquitin-mediated degradation of insulin receptor in obese mice. Diabetes 2017, 66(3):689-698.

  27. Li D-Z, Liu S-F, Zhu L, Wang Y-X, Chen Y-X, Liu J, Hu G, Yu X, Li J, Zhang J: FBXW8-dependent degradation of MRFAP1 in anaphase controls mitotic cell death. Oncotarget 2017, 8(57):97178.

  28. Klaeger S, Heinzlmeir S, Wilhelm M, Polzer H, Vick B, Koenig P-A, Reinecke M, Ruprecht B, Petzoldt S, Meng C: The target landscape of clinical kinase drugs. Science 2017, 358(6367):eaan4368.

  29. Gu S, Chen K, Yin M, Wu Z, Wu Y: Proteomic profiling of isogenic primary and metastatic medulloblastoma cell lines reveals differential expression of key metastatic factors. Journal of Proteomics 2017, 160:55-63.

  30. Wu W-J, Shi J, Hu G, Yu X, Lu H, Yang M-L, Liu B, Wu Z-X: Wnt/β-catenin signaling inhibits FBXW7 expression by upregulation of microRNA-770 in hepatocellular carcinoma. Tumor Biology 2016, 37(5):6045-6051.

  31. Wang Y, Xiao Y, Chen K, Chen S, Zhang M, Wu Z, Wu Y: Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells. Molecular Medicine Reports 2016, 13(3):2724-2728.

  32. Sun M, Xu J, Wu Z, Zhai L, Liu C, Cheng Z, Xu G, Tao S, Ye B-C, Zhao Y: Characterization of protein lysine propionylation in Escherichia coli: global profiling, dynamic change, and enzymatic regulation. Journal of Proteome Research 2016, 15(12):4696-4708.

  33. Shi J, Wu W-J, Hu G, Yu X, Yu G-S, Lu H, Yang M-L, Liu B, Wu Z-X: Regulation of β-catenin transcription activity by leupaxin in hepatocellular carcinoma. Tumor Biology 2016, 37(2):2313-2320.

  34. Nie L, Zhu M, Sun S, Zhai L, Wu Z, Qian L, Tan M: An optimization of the LC-MS/MS workflow for deep proteome profiling on an Orbitrap Fusion. Analytical Methods 2016, 8(2):425-434.

  35. Chen K, Lv F, Xu G, Zhang M, Wu Y, Wu Z: Phosphoproteomics reveals ALK promote cell progress via RAS/JNK pathway in neuroblastoma. Oncotarget 2016, 7(46):75968.

  36. Wu Z, Cheng Z, Sun M, Wan X, Liu P, He T, Tan M, Zhao Y: A chemical proteomics approach for global analysis of lysine monomethylome profiling. Molecular & Cellular Proteomics 2015, 14(2):329-339.

  37. Sun W, Xie Z, Liu Y, Zhao D, Wu Z, Zhang D, Lv H, Tang S, Jin N, Jiang H: JX06 Selectively Inhibits Pyruvate Dehydrogenase Kinase PDK1 by a Covalent Cysteine ModificationSelective and Covalent Inhibition of PDK1. Cancer Research 2015, 75(22):4923-4936.

  38. Médard G, Pachl F, Ruprecht B, Klaeger S, Heinzlmeir S, Helm D, Qiao H, Ku X, Wilhelm M, Kuehne T: Optimized chemical proteomics assay for kinase inhibitor profiling. Journal of proteome research 2015, 14(3):1574-1586.

  39. Lemeer S, Gholami AM, Wu Z, Kuster B: Quantitative proteome profiling of human myoma and myometrium tissue reveals kinase expression signatures with potential for therapeutic intervention. Proteomics 2015, 15(2-3):356-364.

  40. Zhang Y, Wu Z, Wan X, Liu P, Zhang J, Ye Y, Zhao Y, Tan M: Comprehensive profiling of lysine acetylome in Staphylococcus aureus. Science China Chemistry 2014, 57(5):732-738.

  41. Xu G, Wang J, Wu Z, Qian L, Dai L, Wan X, Tan M, Zhao Y, Wu Y: SAHA regulates histone acetylation, butyrylation, and protein expression in neuroblastoma. Journal of proteome research2014, 13(10):4211-4219.

  42. Buecker C, Srinivasan R, Wu Z, Calo E, Acampora D, Faial T, Simeone A, Tan M, Swigut T, Wysocka J: Reorganization of enhancer patterns in transition from naive to primed pluripotency. Cell stem cell 2014, 14(6):838-853.

  43. Gholami AM, Hahne H, Wu Z, Auer FJ, Meng C, Wilhelm M, Kuster B: Global proteome analysis of the NCI-60 cell line panel. Cell reports 2013, 4(3):609-620.

  44. Wu Z, Gholami AM, Kuster B: Systematic identification of the HSP90 regulated proteome. Molecular & cellular proteomics 2012, 11(6).

  45. Lemeer S, Bluwstein A, Wu Z, Leberfinger J, Müller K, Kramer K, Kuster B: Phosphotyrosine mediated protein interactions of the discoidin domain receptor 1. Journal of proteomics 2012, 75(12):3465-3477.

  46. Wu Z, Doondeea JB, Gholami AM, Janning MC, Lemeer S, Kramer K, Eccles SA, Gollin SM, Grenman R, Walch A: Quantitative chemical proteomics reveals new potential drug targets in head and neck cancer. Molecular & Cellular Proteomics 2011, 10(12).

  47. Luo J, Zheng W, Wang Y, Wu Z, Bai Y, Lu Z: Detection method for methylation density on microarray using methyl-CpG-binding domain protein. Analytical biochemistry 2009, 387(2):143-149.

  48. Wu Z, Luo J, Ge Q, Lu Z: Microarray-based Ms-SNuPE: near-quantitative analysis for a high-throughput DNA methylation. Biosensors and Bioelectronics 2008, 23(9):1333-1339.

  49. Wu Z, Luo J, Ge Q, Zhang D, Wang Y, Jia C, Lu Z: Allele-specific extension on microarray for DNA methylation analysis. Analytica chimica acta 2007, 603(2):199-204.