曹木青          

细胞骨架相关细胞器与疾病研究组

电话：021-63846590-776935

邮箱：muqingcao@shsmu.edu.cn

细胞骨架及相关细胞器(如中心体、纤毛等)在形态维持、信号传递、细胞分裂、细胞干性和分化、组织器官发育和稳态维持等众多生命活动中发挥着广泛作用，这些细胞器的异常往往伴随着疾病的发生。本实验室主要使用人类遗传学、动物模型、细胞生物学和结构生物学等手段，探讨细胞骨架相关细胞器在生理和病理生理状态下的调控方式及相关疾病的致病机理，并致力于开发疾病相关的治疗方法。

主要研究方向：

1.中心体和纤毛相关的人类遗传疾病。

2.细胞骨架如何在癌症、再生和衰老等病理生理过程发挥作用。

曹木青，上海交通大学基础医学院病理生理学系PI、细胞分化与凋亡教育部重点实验室PI，获得国自然优青项目、上海高校青年东方学者人才项目支持。主要关注细胞骨架相关细胞器在生理和病理生理过程中的调控方式和作用，使用多种技术手段，探讨相关疾病的发病机理。独立建组后，以通讯作者（含共同通讯）在Genetics in Medicine（2021）、Nature Communications（2022，2023）、PLoS Biology（2023）、EMBO Reports（2023）等学术期刊发表多篇研究论文。

代表性论文(^# co-corresponding author, *co-first author)

1. Xie H*, Kang Y*, Liu J, Huang M, Dai Z, Shi J, Wang S, Li L, Li Y, Zheng P, Sun Y, Han Q, Zhang J, Zhu Z, Xu L, Yelick PC^#, Cao M^#, Zhao C^#. (2023) Ependymal polarity defects coupled with disorganized ciliary beating drive abnormal cerebrospinal fluid flow and spine curvature in zebrafish. PLOS Biology.

2. Zhu P*, Zheng P*, Kong X, Wang S, Cao M^#, Zhao C^#. (2023) Rassf7a promotes spinal cord regeneration and controls spindle orientation in neural progenitor cells. EMBO Reports.

3. Wang L*^#, Wen X*, Wang Z*, Lin Z, Li C, Zhou H, Yu H, Li Y, Cheng Y, Chen Y, Lou G, Pan J, Cao M^#. (2022) Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding. Nature Communications.

4. Qiu YL*, Wang L*, Huang M*, Lian M, Wang F, Gong Y, Ma X, Hao CZ, Zhang J, Li ZD, Xing QH^#, Cao M^#, Wang JS^#. (2022) Association of novel TMEM67 variants with mild phenotypes of high gamma-glutamyl transpeptidase cholestasis and congenital hepatic fibrosis. J Cell Physiol.

5. Huang M*, Kong X*, Tang Z*, Lin Z, He R, Cao M^#, Zhang X^#. (2022) Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly. J Cell Physiol.

6. Luo M*, Lin Z*, Zhu T*, Jin M*, Meng D, He R, Cao Z, Shen Y, Lu C, Cai R, Zhao Y, Wang X, Li H, Wu S, Zou X, Luo G, Cao L, Huang M, Jiao H, Gao H, Sui R^#, Zhao C^#, Ma X^#, Cao M^#. (2021) Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome. Genetics in Medicine.

7. Luo M*, He R*, Lin Z*, Shen Y, Zhang G, Cao Z, Lu C, Meng D, Zhang J^#, Ma X^#, Cao M^#. (2020) Novel Compound Heterozygous Variants in MKS1 Leading to Joubert Syndrome. Front Genet. 11: 576235

8. Luo M*, Cao L*, Cao Z, Ma S, Shen Y, Yang D, Lu C, Lin Z, Liu Z, Yu Y, Cai R, Chen C, Gao H, Wang X, Cao M^#, Ma X^#. (2019) Whole exome sequencing reveals novel CEP104 mutations in a Chinese patient with Joubert syndrome. Mol Genet Genomic Med. 7: e1004

9. Cao M*, Ning J*, Hernandez-Lara CI*, Belzile O, Wang Q, Dutcher SK, Liu Y, Snell WJ. (2015) Uni-directional ciliary membrane protein trafficking by a cytoplasmic retrograde IFT motor and ciliary ectosome shedding. eLife.

10. Meng D*, Cao M*, Oda T, Pan J. (2014) The conserved ciliary protein Bug22 controls planar beating of Chlamydomonas flagella. J Cell Sci.

11. Cao M, Meng D, Wang L, Bei S, Snell WJ^#, Pan J^#. (2013) Activation loop phosphorylation of a protein kinase is a molecular marker of organelle size that dynamically reports flagellar length. PNAS.

12. Wang L*, Piao T*, Cao M, Qin T, Huang L, Deng H, Mao T, Pan J (2013) Flagellar regeneration requires cytoplasmic microtubule depolymerization and kinesin-13. J Cell Sci.

13. Luo M*, Cao M*, Kan Y, Li G, Snell W^#, Pan J^#. (2011) The phosphorylation state of an aurora-like kinase marks the length of growing flagella in Chlamydomonas. Current Biology.

张秀娟，助理研究员

贺睿达，科研助理

林再胜，博士研究生（2020级）

李  琰，博士研究生（2022级）

孔新隆，硕士研究生（2020级）