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Proteomics Laboratory

The proteomics laboratory is closely connected to the research foundation and characteristics of Shanghai Jiao Tong University School of Medicine, with the p...

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Main Instruments
Orbitrap
Principal instrument_5600

Service Items

01Quantitative analysis of proteins

Protein quantitative analysis refers to the qualitative and quantitative analysis of the protein group in the sample using mass spectrometry. Currently, it includes non-targeted relative quantitative analysis and targeted absolute quantitative analysis.

02Qualitative analysis of proteins

​Protein qualitative analysis refers to the identification and sequence analysis of proteins in a sample using mass spectrometry. The most widely used strategy for protein qualitative analysis is "bottom-up", which involves protein digestion by proteases into peptide segments, mass spectrometry analysis of peptide segment information, and the use of software for database retrieval or De Novo a...

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Applications Examples

利用抗体亲和免疫共沉淀偶联质谱技术解析互作蛋白，通过高分辨质谱+PTM质控软件精准鉴定目标蛋白的泛素化位点

利用抗体亲和免疫共沉淀偶联质谱技术解析互作蛋白，通过高分辨质谱+PTM质控软件精准鉴定目标蛋白的泛素化位点

利用化学蛋白质组学技术鉴定可诱导分化早幼粒白血病细胞的小分子药物-腺花素的靶标蛋白并确定其作用位点

利用化学蛋白质组学技术鉴定可诱导分化早幼粒白血病细胞的小分子药物-腺花素的靶标蛋白并确定其作用位点，相关数据发表在Nature Chemical Biology上

Recommended Simplified Mthods


Teams

Assistant Experimentalist Li Zhou Mail: zhouli_bit@shsmu.edu.cn
Tel: 02163846590-776948
Address: Room302, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China
Experimentalist Shuang Meng Mail: mengshuang92@shsmu.edu.cn
Tel: 02163846590-776948
Address: Room302, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China
Experimentalist Liyuan Meng Mail: mengliyuan@shsmu.edu.cn
Tel: 02163846590-776948
Address: Room302, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China
Associate Professor Li Xia, Director Mail: lixia@shsmu.edu.cn
Tel: 02163846590-776948
Address: Room302, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China

Service Guide

2023 Recommended Simplified Mthods more
2023 Research workflow Labelled quantitative analysis ITRAQ/TMTFor protein samples that require labeled quantitation, after extracting high-quality proteins, isotopic labeling is required for the samples. The labeling reagents can be selected based on the number of samples (iTRAQ: 2-8 samples, TMT: 2-10 samples).Label-free quantificationFor label-free quantitation (LFQ) analysis in proteomics, there are two main meth... more
2023 Differential quantification Differential quantitation based on proteomics is similar in purpose to transcriptome chips and RNA-Seq. The goal is to identify differences in protein expression levels between two or more different states, times, or varieties of samples. This information is used to correlate and further study the differences in phenotype. This is currently the most widely used and closely integrated technical ... more
2023 Sample preparation Freshly collected samples are frozen directly in a -80°C freezer.Once the sample is collected, send it with dry ice. Be careful to avoid repeated freeze-thaw of samples throughout the process of cryo-preservation and shipping to prevent protein degradation more
2023 Sample source Samples from a variety of sources can be analyzed with no special requirements as to thesourceof the sample. Such as model animals, tissues, cultured cells, body fluid samples, plants, microorganisms, etc., can be analyzed in proteomics more

Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine

postcode 299925

Old Red Mansion, 280 Chongqing South Road, Shanghai, China

021-63846590x776946

ICP record No. HJY ICP B 117,001