Laboratory of Protein Crystallography-Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medic

Laboratory of Protein Crystallography

The Laboratory of Protein Crystallography offers technical services for related disciplines and research groups both inside and outside the School of Medicin...

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Main Instruments

各种蛋白分离层析柱及填料

CHIRASCAN v100圆二色光谱仪

Agilent Seahorse XFe24细胞能量代谢分析仪

Gator非标记生物分子分析仪

ChemiDoc XRS+化学发光成像系统

AKTA purifier 10 / 100快速蛋白纯化系统

Service Items

Cell analysis Rapid detection of various fluorescein labeled cells by flow cytometry

01In vitro recombinant protein preparation

Detection services of expression plasmidsSoluble protein expression and supernatant optimizationProtein purification servicesInclusion body renaturation servicesMolecular interaction detectio

02Protein Crystallization

Protein identificationHigh-throughput screening of protein crystal growth conditionsCrystal culture condition optimization and single crystal cultur

03Diffraction data collection and processing

High-resolution X-ray diffraction data collection of protein crystalsThree-dimensional data analysis of crystal dat

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Applications Examples

糖酵解速率分析

细胞线粒体功能分析

动力学 (K): 测定杂交瘤细胞上清液中的抗体亚型

动力学 (K): 测定杂交瘤细胞上清液中的抗体亚

动力学 (K)-亲和力测定

动力学 (K)-亲和力测

蛋白质三级结构的热变性过程和热稳定性研究

蛋白质的二级结构和三级结构测定

蛋白质的二级结构和三级结构测

凝胶过滤层析

Ni亲和纯化

Our Achievements

1. The role of AGK in thrombocytopoiesis and possible therapeutic strategies .Blood .(2020)

2.Branched-Chain Amino Acid Catabolism Promotes Thrombosis Risk byEnhancing Tropomodulin-3 Propionylation in Platelets. Circulation. (2020)

3.Metabolic imaging reveals a unique preference of symmetric cell division and homing of leukemia-initiating cells in an endosteal niche . Cell Metabolism . (2019)

4.Luteinizing hormone signaling restricts hematopoietic stem cell expansion during puberty. EMBO J. (2018)

5.JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling . J Clin Invest. (2018)

6.Leukaemic alterations of IKZF1 prime stemness and malignancy programs in human lymphocytes. Cell Death Dis. (2018)

7.Vacuolar Protein Sorting 33B Is a Tumor Suppressor in Hepatocarcinogenesis. Hepatology. (2018)

8.Expression of the fetal hematopoiesis regulator FEV indicates leukemias of prenatal origin. Leukemia. (2017)

9.Platelet-Specific p38α Deficiency Improved Cardiac Function After Myocardial Infarction in Mice. Arterioscler Thromb Vasc Biol.(2017)

10.Sorting protein VPS33B regulates exosomal autocrine signaling to mediate hematopoiesis and leukemogenesis. J Clin Invest. (2016)

Teams

Senior Experimentalist ZhenQuan Wei, Direcotor Mail: zhenquan.wei@sjtu.edu.cn
Tel: 02163846590-776964
Address: Room6215, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China

Senior Experimentalist Lingling Feng Mail: fenglingling83@163.com
Tel: 02163846590-776964
Address: Room6215, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China

Service Guide

2023 Sorting receiver tube requirements 1. When sorting, double-concentration double-antibotics culture medium should be placed in the sterile receiving tube to prevent contamination;2. The amount of culture medium added in the receiving tube does not make specific requirements, if the amount of cells to be sorted is large, about 1ml can be added; If the amount of cells to be sorted is small, 3ml or more can be added to reduce the fo... more

2023 Sorting sample requirements 1. Sorting cells must ensure the whole process of sterile operation, this platform does not accept bacterial samples, please filter the cells in a biosafety cabinet with a sterile filter membrane (usually 40 μm) before putting on the machine;2. Loading cell concentration: Aria II. 2×10^7/mL, Moflo 2~4×10^7/mL;3. Be sure to centrifuge immediately after the end of sorting to remove the supernata... more

Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine

postcode 299925

Old Red Mansion, 280 Chongqing South Road, Shanghai, China

021-63846590x776946

FriendLinks: Shanghai Jiaotong University School of Medicine

ICP record No. HJY ICP B 117,001