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Flow Cytometry Laboratory

​Flow cytometry is a powerful tool in immunology, cancer research, stem cell research, drug development, and molecular biology.In immunology, flow cytometry...

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Main Instruments
BD FACSAria III 高速流式细胞分选仪
​Cell sorter BD FACSAria llu
BD FACSymphony™ A5 流式细胞分析仪
Beckman Coulter CytoFLEX LX 流式细胞分析仪
BD LSRFortessa X-20流式细胞分析仪
Cell Analyzer Beckman Coulter CytoFlex S

Service items

Rapid detection of a wide range of fluorescein-labeled cells by flow cytometry

01Immunology related experiments

Lymphocytes subtype, functional analysis, cell activation, cytokine detection, immune cell leukocyte differentiation antigen detection, leukemia immunophenotyping, patient immune status monitoring after organ transplantation and bone marrow transplantation, platelet function and related diseases, regulatory T cell Foxp3 preparation and detection analysi

02Cell biology related experiments

Cell size, cell viability, cell morphology, apoptosis and apoptosis-related proteins, transfection & gene silencing efficiency assay, cell cycle and ploidy analysis, macrophage phagocytosis assay, exosome assay, intracellular pH, calcium ion concentration determination, reactive oxygen species assay, membrane potential, mitochondrial membrane potential, cytoskeletal components, telomere lengt

03High-purity target cells are rapidly sorted from complex cell populations by high-speed flow cytometry

Multi-color labeled immune cell subset sorting, cancer stem cell sorting, hematopoietic stem cell sorting, GFP-positive cell sorting, lateral population SP cell sorting, etc., to obtain high-activity, high-purity target cells, continue to be sterile culture, or used for downstream PCR, RNA-SEQ, FISH and other experiment

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Applications Examples

淋巴细胞亚群分选

淋巴新报亚群分选NaiveT cell和effector.memory T cell回测纯度发现，分选细胞纯度都可达98%以上

胚胎发布16.5天的肝脏造血干祖细胞分选回测纯度发现分选细胞纯度可达95%以上

胚胎发布16.5天的肝脏造血干祖细胞分选回测纯度发现分选细胞纯度可达95%以上

9色淋巴细胞亚型分析

9色淋巴细胞亚型分析

Hoechst 33342染色检测DNA倍体

Hoechst 33342染色检测DNA倍体

Our Achievements

1. The role of AGK in thrombocytopoiesis and possible therapeutic strategies .Blood .(2020)

2.Branched-Chain Amino Acid Catabolism Promotes Thrombosis Risk byEnhancing Tropomodulin-3 Propionylation in Platelets. Circulation. (2020)

3.Metabolic imaging reveals a unique preference of symmetric cell division and homing of leukemia-initiating cells in an endosteal niche . Cell Metabolism . (2019)

4.Luteinizing hormone signaling restricts hematopoietic stem cell expansion during puberty. EMBO J. (2018)

5.JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling . J Clin Invest. (2018)

6.Leukaemic alterations of IKZF1 prime stemness and malignancy programs in human lymphocytes. Cell Death Dis. (2018)

7.Vacuolar Protein Sorting 33B Is a Tumor Suppressor in Hepatocarcinogenesis. Hepatology. (2018)

8.Expression of the fetal hematopoiesis regulator FEV indicates leukemias of prenatal origin. Leukemia. (2017)

9.Platelet-Specific p38α Deficiency Improved Cardiac Function After Myocardial Infarction in Mice. Arterioscler Thromb Vasc Biol.(2017)

10.Sorting protein VPS33B regulates exosomal autocrine signaling to mediate hematopoiesis and leukemogenesis. J Clin Invest. (2016)

Teams

Experimentalist Chenzhi Guo, Director Mail: guochenzhi@shsmu.edu.cn
Tel: 02163846590-776964
Address: Room215, Building1,Old Red Mansion, 280 Chongqing South Road, Shanghai, China
Experimentalist Xiaocui Zhang, Phd Mail：zhangcui1986921@163.com
Tel：02163846590-776964
Address: Room215, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China
Assistant Experimentalist Nai’an Shen Mail：13761790848@163.com
Tel：02163846590-776964
Address: Room215, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China
Experimentalist Rong Fu Mail：furong@shsmu.edu.cn
Tel：02163846590-776964
Address: Room215, Building1, Old Red Mansion, 280 Chongqing South Road, Shanghai, China

Service Guide

2023 Sorting receiver tube requirements 1. When sorting, double-concentration double-antibotics culture medium should be placed in the sterile receiving tube to prevent contamination;2. The amount of culture medium added in the receiving tube does not make specific requirements, if the amount of cells to be sorted is large, about 1ml can be added; If the amount of cells to be sorted is small, 3ml or more can be added to reduce the fo... more
2023 Sorting sample requirements 1. Sorting cells must ensure the whole process of sterile operation, this platform does not accept bacterial samples, please filter the cells in a biosafety cabinet with a sterile filter membrane (usually 40 μm) before putting on the machine;2. Loading cell concentration: Aria II. 2×10^7/mL, Moflo 2~4×10^7/mL;3. Be sure to centrifuge immediately after the end of sorting to remove the supernata... more

Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine

postcode 299925

Old Red Mansion, 280 Chongqing South Road, Shanghai, China

021-63846590x776946

ICP record No. HJY ICP B 117,001