1. Sorting cells must ensure the whole process of sterile operation, this platform does not accept bacterial samples, please filter the cells in a biosafety cabinet with a sterile filter membrane (usually 40 μm) before putting on the machine;

2. Loading cell concentration: Aria II. 2×10^7/mL, Moflo 2~4×10^7/mL;

3. Be sure to centrifuge immediately after the end of sorting to remove the supernatant, and replace the cells with fresh culture medium (double-concentration double-antibotics culture medium for the first week, and then change to normal culture medium for culture).