Flow Cytometry Laboratory
Flow cytometry is a powerful tool in immunology, cancer research, stem cell research, drug development, and molecular biology.In immunology, flow cytometry...
Service items
Rapid detection of a wide range of fluorescein-labeled cells by flow cytometry
Applications Examples
淋巴细胞亚群分选
淋巴新报亚群分选NaiveT cell和effector.memory T cell回测纯度发现，分选细胞纯度都可达98%以上
胚胎发布16.5天的肝脏造血干祖细胞分选回测纯度发现分选细胞纯度可达95%以上
胚胎发布16.5天的肝脏造血干祖细胞分选回测纯度发现分选细胞纯度可达95%以上
9色淋巴细胞亚型分析
9色淋巴细胞亚型分析
Hoechst 33342染色检测DNA倍体
Hoechst 33342染色检测DNA倍体
Sorting receiver tube requirements
1. When sorting, double-concentration double-antibotics culture medium should be placed in the sterile receiving tube to prevent contamination;
2. The amount of culture medium added in the receiving tube does not make specific requirements, if the amount of cells to be sorted is large, about 1ml can be added; If the amount of cells to be sorted is small, 3ml or more can be added to reduce the force of cells rushing down from the machine and reduce the damage to cells;
3. If the receiving tube is a 5mL flow cytometry tube, it can receive about 3×10^6 cells, if the receiving tube is a 15mL centrifuge tube, it can receive about 1×10^7 cells.
Note: The concentration of double penicillin (Penicillin): 200 units/mL, and the concentration of double streptomycin (Streptomycin) is 200 μg/mL
Service Guide
Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine
postcode 299925
Old Red Mansion, 280 Chongqing South Road, Shanghai, China
021-63846590x776946
