FBS

Imaging Laboratory

Imaging Laboratory has six large optical microscopes, including super-resolution microscope, confocal laser scanning microscope and two-photon microscope, wh...

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Main Instruments

Service Items

01Multiphoton laser scanning microscopy

animalinvivo imaing, deep tissue imagingCalcium biology researchNeurological researchEmbryology and histology studiesMetabolic process researc

02Confocal laser scanning microscopy

Sample image tomographyLive cell dynamic image acquisition3D image acquisition and reconstructionQuantitative fluorescence analysisFluorescence colocalization analysisCalcium imagingFRAP (Fluorescence Recovery after Photobleaching) experimentFRET (fluorescence resonance energy transfer) experimen

03Super-resolution fluorescence microscopy

Sample image tomographyLive cell dynamic image acquisition3D image acquisition and reconstructionQuantitative fluorescence analysisFluorescence colocalization analysisCalcium imagingFRAP (Fluorescence Recovery after Photobleaching) experimentFRET (fluorescence resonance energy transfer) experimen

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Applications Examples

Multiphoton laser scanning microscopy

Confocal Laser scanning microscopy

Super-resolution fluorescence microscopy

有丝分裂过程

有丝分裂过程

植物花粉粒三维重构

植物花粉粒三维重构

拟南芥根

拟南芥根

培养的神经元细胞

​培养的神经元细

小鼠脑片标本 标本厚度: 300um

小鼠脑片标本标本厚度: 300u

小鼠胰腺 血管标记 Texas Red–labeled lectin

小鼠胰腺 血管标记Texas Red–labeled lecti

细胞核孔复合体

细胞核孔复合体

小鼠神经元轴突蛋白Alexa594标记

小鼠神经元轴突蛋白Alexa594标记

Our Achievements

1.The role of AGK in thrombocytopoiesis and possible therapeutic strategies .Blood .(2020)

2.Branched-Chain Amino Acid Catabolism Promotes Thrombosis Risk byEnhancing Tropomodulin-3 Propionylation in Platelets. Circulation. (2020)

3.Lgr4 Governs a Pro-Inflammatory Program in Macrophages to Antagonize Post-Infarction Cardiac Repair. Circulation research.(2020)

4.Metabolic imaging reveals a unique preference of symmetric cell division and homing of leukemia-initiating cells in an endosteal niche . Cell Metabolism . (2019)

5.Enhanced Drug Delivery by Nanoscale Integration of a Nitric Oxide Donor To Induce Tumor Collagen Depletion . Nano Lett . (2018)

6.A neuronal molecular switch through cell-cell contact that regulates quiescent neural stem cells . Science Advances . (2018)

7.TRPV1 SUMOylation regulates nociceptive signaling of inflammatory pain. Nat Commun. (2018)

8.JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling. J Clin Invest. (2018)

9.Nuclear PTEN safeguards pre-mRNA splicing to link Golgi apparatus for its tumor suppressive role . Nat Commun .(2018)

10.Vacuolar Protein Sorting 33B Is a Tumor Suppressor in Hepatocarcinogenesis. Hepatology. (2018)

11.Neuronal GAP-Porf-2 transduces EphB1 signaling to brake axon growth. Cell Mol Life Sci. (2018)

12.Retrograde regulation of mossy fiber axon targeting and terminal maturation via postsynaptic Lnx1. J Cell Biol. (2018)

13.ASIC1a mediates striatal synapse remodeling and procedural motor learning.Sci Signal. (2018)

14.ASIC1a regulates insular long-term depression and is required for the extinction of conditioned taste aversion. Nat Commun. (2016)

15.Ephrin-B3 coordinates timed axon targeting and amygdala spinogenesis for innate fear behavior.Nat Commun. (2016)

16.A redox mechanism underlying nucleolar stress sensing by nucleophosmin.Nat Commun. (2016)

Teams

Service Guide

Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine

postcode 299925

Old Red Mansion, 280 Chongqing South Road, Shanghai, China

021-63846590x776946