FBS

Proteomics Laboratory

The proteomics laboratory is closely connected to the research foundation and characteristics of Shanghai Jiao Tong University School of Medicine, with the p...

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Main Instruments

Service Items

01Quantitative analysis of proteins

Protein quantitative analysis refers to the qualitative and quantitative analysis of the protein group in the sample using mass spectrometry. Currently, it includes non-targeted relative quantitative analysis and targeted absolute quantitative analysis.

02Qualitative analysis of proteins

​Protein qualitative analysis refers to the identification and sequence analysis of proteins in a sample using mass spectrometry. The most widely used strategy for protein qualitative analysis is "bottom-up", which involves protein digestion by proteases into peptide segments, mass spectrometry analysis of peptide segment information, and the use of software for database retrieval or De Novo a...

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Applications Examples

利用抗体亲和免疫共沉淀偶联质谱技术解析互作蛋白,通过高分辨质谱+PTM质控软件精准鉴定目标蛋白的泛素化位点

利用抗体亲和免疫共沉淀偶联质谱技术解析互作蛋白,通过高分辨质谱+PTM质控软件精准鉴定目标蛋白的泛素化位点

利用化学蛋白质组学技术鉴定可诱导分化早幼粒白血病细胞的小分子药物-腺花素的靶标蛋白并确定其作用位点

利用化学蛋白质组学技术鉴定可诱导分化早幼粒白血病细胞的小分子药物-腺花素的靶标蛋白并确定其作用位点,相关数据发表在Nature Chemical Biology上

Orbitrap

Thermo Scientific™ LTQ Orbitrap XL™ uses LTQ Orbitrap technology to provide breakthrough technology services for various laboratories. With its powerful functionality, stability, and low operating costs, it has become the best choice for proteomics and metabolomics applications, completely surpassing and replacing Q-TOF systems. With its high-resolution and precise mass analysis capabilities, the hybrid mass spectrometry system achieves high-throughput label-free identification of differential expression. The unparalleled MS and MSn sensitivity, fast scan rate, high quality accuracy, and high resolution capabilities of the LTQ Orbitrap XL make it an ideal choice for protein identification and biomarker research.

Application Field and Characteristics of The Instrument:

Protein Identification

• High mass accuracy lowers false positive rates

• Parallel acquisition mode optimizes productivity

• High MS and MS/MS sensitivity, wide dynamic range and fast cycle times increase protein identifications

Biomarker Discovery

• HCD collision cell provides high fragmentation efficiency combined with high sensitivity for the reporter and fragment ions of the peptide sequence

• Reliable identification and quantification in one experiment

Teams

Service Guide

Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine

postcode 299925

Old Red Mansion, 280 Chongqing South Road, Shanghai, China

021-63846590x776946