1. When sorting, double-concentration double-antibotics culture medium should be placed in the sterile receiving tube to prevent contamination;

2. The amount of culture medium added in the receiving tube does not make specific requirements, if the amount of cells to be sorted is large, about 1ml can be added; If the amount of cells to be sorted is small, 3ml or more can be added to reduce the force of cells rushing down from the machine and reduce the damage to cells;

3. If the receiving tube is a 5mL flow cytometry tube, it can receive about 3×10^6 cells, if the receiving tube is a 15mL centrifuge tube, it can receive about 1×10^7 cells.

Note: The concentration of double penicillin (Penicillin): 200 units/mL, and the concentration of double streptomycin (Streptomycin) is 200 μg/mL