1. Purpose:

In order to standardize the good safety behavior and work norms of laboratory staff and better serve customers, these regulations are specially formulated.

2. Scope:

All personnel entering and exiting each laboratory must comply with this.

3. Contents:

3.1 General rules for basic laboratory safety:

3.1.1 All items in the laboratory must be managed and inspected in accordance with relevant regulations, and strict procedures for collection shall be established, and radioactive drugs, highly toxic substances and valuable reagents shall be managed by double locks to prevent the loss of items.

3.1.2 A large number of chemical dangerous goods should not be stored in the laboratory, chemical reagents should be classified and stored in isolation, and gas cylinders should be managed and used according to the nature of the gas. In the storage room and laboratory, eye-catching marks such as "no smoking fire" should be affixed, and fire extinguishing equipment should be installed at the entrance of the laboratory for easy access.

3.1.3 Before carrying out experimental operations, you must be familiar with the correct experimental operation procedures and instrument operating procedures, and understand the possible dangers, safety measures and emergency treatment steps of the instruments, biological samples and chemicals to be used. If several items or methods can achieve the purpose of the experiment, the safer items or methods are selected.

3.1.4 When the instrument fails, it should be indicated in the record book and notified to the relevant responsible personnel for maintenance, so as to avoid others from using it without their knowledge, causing danger and aggravating the damage of the instrument.

3.1.5 Comply with the requirements for wearing protective equipment in the laboratory, wear work clothes, protective glasses and protective gloves, etc. Make it a habit to follow safety regulations, prohibit wearing open-toed shoes in the laboratory, long hair tied behind your head, and do not wear accessories that could be involved in the instrument.

3.1.6 After the end of the experiment, the items should be returned to their places, and safety inspections should be carried out, and all power supplies, water sources, gas sources, etc. should be turned off.

3.2 Laboratory reagents and sample storage requirements:

Most of the chemical reagents have certain toxicity and danger, and the management of chemical reagents is not only the need to ensure the quality of analysis results, but also the need to ensure the safety of life and property. The management of chemical reagents should be properly managed in different ways according to different characteristics.

3.2.1 Highly toxic drugs should be locked in a special drug cabinet.

3.2.2 Flammable and explosive materials should be stored separately in a cool and ventilated place, or stored in an iron cabinet.

3.2.3 Organic solvents should be stored in a fireproof cabinet under the fume hood.

3.2.4 Radioactive chemicals shall be stored in lead vessels.

3.2.5 Chemically active reagents are stored in refrigerators and dryers.

3.2.6 Strong corrosive reagents should be placed on shelves made of anti-corrosive materials, or stored on a cool and ventilated ground against the wall.

3.2.7 Strong oxidants should be stored in a cool and ventilated place, and should be stored separately from organic matter.

3.2.8 Valuable reagents should be stored separately from general reagents to strengthen management.

3.2.9 After checking and registering biological products and biological samples, they must be placed in the designated area in strict accordance with the conditions required for preservation and placed separately from other reagents.

3.2.10 Inorganic salts and daily laboratory supplies (non-reagent laboratory supplies) can be stored in a glass cabinet on the countertop.

3.3 Principle of small solvent storage:

Organic solvents are a major safety hazard in the laboratory, all staff do not hinder the test process, as little as possible to store organic solvents in the laboratory, the specific principles are as follows:

3.3.1 In principle, bottled solvents used by laboratories will be received on a daily basis. FOR GENERAL SOLVENTS, THE NUMBER OF STORAGE PER PERSON SHALL NOT EXCEED 10 BOTTLES (INCLUDING SPECIFICATIONS LESS THAN 500ML/BOTTLE).

3.3.2 Commonly used solvents acetone, ethyl acetate, petroleum ether, dichloromethane, tetrahydrofuran, the total amount of solvent storage in each laboratory shall not exceed 30L.

3.4 Safety precautions for the use of highly toxic chemicals:

3.4.1 When using highly toxic chemicals, at least two people work together, take personal protective measures, and wear gloves and face shields.

3.4.2 All operations that can produce toxic gases must be carried out in fume hoods (common toxic gases are sulfuric acid smoke, halogen steam, hydrochloric acid vapor, ammonia, nitric acid and nitrogen oxides, sulfides, carbon monoxide, mercury vapor, etc.).

3.4.3 Mercury spillage, should be carefully cleaned and collected, and then sprinkled with sulfur powder on the residue to eliminate mercury droplets.

3.4.4 The smell of olfactory examination reagents and reactions should not be directly close to the container, only a small amount of gas can be sent by hand fan, sniffing gently, and oral taste is strictly prohibited. When using pipettes or pipettes to aspirate liquids, mouth aspiration.

3.5 Safety precautions for the use of flammable and explosive materials:

3.5.1 When using flammable and explosive materials, it should be operated according to its characteristics, and at least two people should operate together to reduce the occurrence of personal injury accidents and fires.

3.5.2 Explosive materials should be handled gently, scattered powders and particles should be collected gently, and collision impact is strictly prohibited.

3.5.3 Use peroxide, prohibit impact and prevent exposure to air.

3.5.4 Cyanide is strictly forbidden to be mixed with acid, once a fire occurs, acid and alkali fire extinguishers, foam fire extinguishers can not be used, sand can be used to extinguish fires, and gas masks must be worn when extinguishing fires.

3.5.5 When using flammable reagents, it must be carried out in a fume hood with flame retardant structure, it is strictly forbidden to be close to the fire source, and it must be sealed immediately after use and stored in a cool and ventilated place.

3.5.6 If the boiling point of the flammable solution is below 60 °C, use isolated fire source equipment (such as constant temperature water bath, etc.) for heating during recovery; If the boiling point is between 60~100 °C, a water bath should be used; An oil bath should be used with a boiling point above 100 °C.

3.5.7 Flammable wastes shall be recycled or destroyed in open ventilation places and shall not be poured into sewers.

3.6 Safety precautions for the use of strong acids and alkalis:

3.6.1 When using strong acids and alkalis, the operation should be careful, avoid spilling or splashing, and wear protective gloves and masks.

3.6.2 When diluting concentrated acid, the acid must be slowly poured into the water and stirred at the same time to avoid sudden heat causing the acid to splash out at night or the container to break, and accidents will occur.

3.6.3 The use of strong alkalis and reagents with strong irritating odor should be carried out in a fume hood.

3.7 Safety precautions for the use of electrical equipment:

3.7.1 When installing various electrical appliances, the ground wire should be connected, the specifications of the fuse shall not be arbitrarily changed, and copper wire, iron wire or other metals shall be prohibited instead.

3.7.2 Before using electrical equipment, use the electric pen to check whether there is leakage, and if it is found that the electrical wire leakage or the insulator is damaged and the plug and switch are broken, the use should be stopped immediately, and the instrument management personnel should be notified in time.

3.7.3 When using various electrical appliances, they should be operated in accordance with standard operating procedures and should not be moved to avoid accidents.

3.7.4 When pushing and pulling the electric gate, do not face the electric gate to avoid electric sparks hurting the eyes.

3.7.5 When the instrument is repaired, the power supply must be disconnected.

3.7.6 When checking whether electrical equipment is hot, the shell should be tested with the back of the hand, and the palm surface should not be used to touch the case, so as to avoid the danger of electric shock.

3.7.7 In the event of electric shock, the power supply should be quickly cut off, or the wire should be picked open with a wooden rod or other insulation, and rescue should be carried out in time.

3.7.8 Do not scrub electrical equipment with water and damp cloths.

3.7.9 When using pressure vessels and equipment, during the operation, there should be a special person to supervise, and the pot (door) can only be opened after the pressure is drained, and it is strictly forbidden to open the pot (door) with pressure.

3.8 Safety precautions for using glass instruments:

3.8.1 Wear special gloves for breaking glass tubes or connecting glass devices.

3.8.2 The glass instrument should be carefully checked before use, and cracks cannot be used.

3.9 Other precautions during experimental operations:

3.9.1 During the experimental operation, you should concentrate your energy, and you should not chat or leave your post without authorization, let alone operate in violation of regulations.

3.9.2 Electric sparks must be avoided in the laboratory, and it is strictly forbidden to use fire and smoke without permission.

3.9.3 When handling chemicals, you must wear protective glasses, once splashed into the eyes, you should immediately rinse with plenty of water, and then seek medical attention, as far as possible to bring the MSDS of the chemical to facilitate the guidance of the doctor for treatment.

3.9.4 It is strictly forbidden to store reagents without lids in the refrigerator.

4. Storage and disposal of laboratory waste:

Domestic waste, chemical dangerous goods waste, medical waste, radioactive waste, etc. generated by the laboratory must be collected separately, and a special person is responsible for sending it to the relevant temporary storage point for disposal. Waste products should not be dumped elsewhere, and it is strictly forbidden to dump waste liquid directly into the sewer.

4.1 Separate collection of waste liquid and solid waste:

4.1.1 Chemical dangerous goods received by the dangerous goods warehouse shall be collected uniformly after use, and transferred to the temporary storage office of the warehouse of the material management center. Other reagent bottles, plastic products, and inorganic residues must be packed in yellow special packaging bags and poured into special garbage cans.

4.1.2 Radioactive waste shall be transferred to the radioisotope platform staging area.

4.1.3 Damaged waste should be collected in a special sharps box.

4.1.4 Infectious wastes in biological samples must be sterilized and disinfected to ensure that they are non-infectious and non-polluting before they can be collected and treated.

4.1.5 Pathological wastes in biological samples must be transferred to the Department of Animal Science for disposal in leak-proof, closed, plastic bags or containers.

4.1.6 Waste liquid, including halogen-containing organic waste liquid, non-halogen-containing organic waste liquid, acid waste liquid and alkaline waste liquid, is classified into a sealed container, and a special person is responsible for transferring it to the temporary storage office of the warehouse of the material management center.

4.1.7 Organic residues must be dissolved and poured into the corresponding waste liquid barrel for collection.

4.2 Responsible person for waste collection and its responsibilities:

4.2.1 Supervisors at all levels have the obligation to supervise and manage the dumping of waste in the laboratory.

4.2.2 Each laboratory designates a special person on duty to be responsible for sorting waste and placing it in the designated waste dump place for regular disposal.

5. Laboratory safety and environmental hygiene inspection:

5.1 In order to ensure the safe and orderly work of the platform, ensure a clean and civilized image, and prevent the occurrence of various accidents, the platform director or platform safety officer shall organize irregular safety, fire protection and hygiene inspections.

5.2 Through irregular patrol inspection, correct the non-compliance with the standards on the spot, fill in the accident hidden danger report form for major rectification matters, and rectify within a time limit.

5.2.1 The general dirty phenomenon should be corrected immediately, and the tracking period that is difficult and needs to be completed through the rectification period is one week; Special reasons that cannot be completed within one week may be included in the two-week follow-up after approval by the Safety Committee

5.2.2 Fire safety problems should be tracked until they are resolved.

6. Other provisions:

6.1 Each laboratory shall regularly organize operators to learn safety operation rules, conduct safety awareness education and safety knowledge training, and prevent accidents.

6.2 For the safe use of chemical substances, please consult the relevant safety data sheets.

6.3 The laboratory shall be kept clean and tidy, it is forbidden to eat and drink in the experimental area, and it is not allowed to use experimental vessels to contain food.

6.4 Work clothes should be placed in the designated area after work every day, and the platform will hire a special person to clean and disinfect them every week.