- Biography
- Education & Experience
- Publications
- Lab Member
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Florent Ginhoux graduated in Biochemistry from the University Pierre et Marie CURIE (UPMC), Paris VI, obtained a Master’s degree in Immunology from the Pasteur Institute in 2000 and his PhD in 2004 from UPMC, Paris VI. As a postdoctoral fellow, he joined the Laboratory of Miriam Merad in the Mount Sinai School of Medicine (MSSM), New York. In 2008, he became an Assistant Professor in the Department of Gene and Cell Medicine, MSSM and member of the Immunology Institute of MSSM. He joined the Singapore Immunology Network (SIgN), A*STAR in May 2009 as a Junior Principal Investigator. He is now a Senior Principal Investigator and an EMBO Young Investigator and his laboratory is focusing on the ontogeny and differentiation of macrophages and dendritic cells (DCs) in both humans and mice. In 2015, he joined Shanghai Institute of Immunology as an adjunct professor.
Dendritic cells, monocytes and macrophages play crucial and distinct roles in tissue homeostasis and immunity, but also contribute to a broad spectrum of pathologies and are thus attractive therapeutic targets. Potential intervention strategies aiming at manipulation of these cells will require in-depth insights of their origins and the mechanisms that govern their homeostasis.
The focus of the laboratory is to understand the ontogeny of DCs, monocytes and macrophages, their differentiation pathways and how their unique ontogeny dictates their immune functions.
Our approach encompasses the integration of high dimensional platforms such as single-cell RNA sequencing and deep immunophenotypic assessment using state of the art 25 parameters flow cytometry. Such high-density molecular profiling at the single level and at unprecedented dimensionality and complexity will provide new insights in the biology of DC, monocyte and macrophage cell populations. Defining macrophage and DC populations on the criteria of their origin may aid our understanding of their discrete roles in tissue immunity and homeostasis, as ontogeny of DC and macrophage subsets likely underlie their functional specializations.
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Education and experience
Present:Senior Principal Investigator, Singapore Immunology Network (SIgN), Singapore
May 2009/March 2014:Junior Principal Investigator, Singapore Immunology Network (SIgN), Singapore
July 2008/April 2009:Assistant Professor, Department of Gene and Cell Medicine and The Immunology Institute，Mount Sinai School of Medicine, New York
Jan.2007/June 2008:Instructor, Dept of Gene and Cell Medicine, Mount Sinai School of Medicine, New York
Sept. 2004/Dec.2006:Postdoctoral fellow, Dept of Gene and Cell Medicine, Mount Sinai School of Medicine, New York
June 2004: Ph.D. in Immunology,Immunology Unit, GENETHON, CNRS UMR 8115, University of Paris VI
2000:Master of Advanced Studies in Fundamental Immunology,PASTEUR Institute, University Pierre et Marie CURIE (Paris VI)
1997-1999:Bachelor Degree, Master Degree and Certificate of Biochemistry,Faculty of Science, University Pierre et Marie CURIE (Paris VI)
1.See P, Dutertre CA, Chen J, Günther P, McGovern N, Irac SE, Gunawan M, Beyer M, Händler K, Duan K, Sumatoh HRB, Ruffin N, Jouve M, Gea-Mallorquí E, Hennekam RCM, Lim T, Yip CC, Wen M, Malleret B, Low I, Shadan NB, Fen CFS, Tay A, Lum J, Zolezzi F, Larbi A, Poidinger M, Chan JKY, Chen Q, Renia L, Haniffa M, Benaroch P, Schlitzer A, Schultze JL, Newell EW, Ginhoux F. ,Mapping the human DC lineage through the integration of high-dimensional techniques.,Science. ,2017 ,Jun 9;356(6342)..[Link]
2.McGovern N, Shin A, Low G, Low D, Duan K, Yao LJ, Msallam R, Low I, Shadan NB, Sumatoh HR, Soon E, Lum J, Mok E, Hubert S, See P, Kunxiang EH, Lee YH, Janela B, Choolani M, Mattar CNZ, Fan Y, Lim TKH, Hong DK, Tan KK, Tam JKC, Schuster C, Elbe-Bürger A, Wang XN, Bigley V, Collin M, Haniffa M, Schlitzer A, Poidinger M, Albani S, Larbi L, Newell EW, Chan JKY, Ginhoux F. ,Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2.,Nature.,2017 ,Jun 29;546(7660):662-666..[Link]
3.Takata K, Kozaki T, Lee CZW, Thion MS, Otsuka M, Lim S, Utami KH, Fidan K, Park DS, Malleret B, Chakarov S, See P, Low D, Low G, Garcia-Miralles M, Zeng R, Zhang J, Goh CC, Gul A, Hubert S, Lee B, Chen J, Low I, Shadan NB, Lum J, Wei TS, Mok E, Kawanishi S, Kitamura Y, Larbi A, Poidinger M, Renia L, Ng LG, Wolf Y, Jung S, Önder T, Newell E, Huber T, Ashihara E, Garel S, Pouladi MA, Ginhoux F. ,Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function.,Immunity. ,2017 ,Jul 18;47(1):183-198.e6..[Link]
4.Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG. ,Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions., Immunity. ,2018 ,Feb 20;48(2):364-379.e8..[Link]
5.inhoux F, Garel S. ,The mysterious origins of microglia.,Nat Neurosci. ,2018 ,Jul;21(7):897-899.[Link]
6.Ginhoux F, Guilliams M. ,Editorial for Cellular Immunology special issue on "Tissue Macrophages".,Cell Immunol,2018 ,Aug;330:1-4..[Link]
7.Silvin A, Ginhoux F. ,Microglia heterogeneity along a spatio-temporal axis: More questions than answers.,Glia., 2018 ,Oct;66(10):2045-2057..[Link]
8.Lee CZW, Kozaki T, Ginhoux F. ,Studying tissue macrophages in vitro: are iPSC-derived cells the answer?,Nat Rev Immunol. ,2018 ,Nov;18(11):726..[Link]
9.Chakarov S, Lim HY, Tan L, Lim SY, See P, Lum J, Zhang XM, Foo S, Nakamizo S, Duan K, Kong WT, Gentek R, Balachander A, Carbajo D, Bleriot C, Malleret B, Tam JKC, Baig S, Shabeer M, Toh SES, Schlitzer A, Larbi A, Marichal T, Malissen B, Chen J, Poidinger M, Kabashima K, Bajenoff M, Ng LG, Angeli V, Ginhoux F. ,Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches.,Science. ,2019 ,Mar 15;363(6432)..[Link]
10.Janela B, Patel AA, Lau MC, Goh CC, Msallam R, Kong WT, Fehlings M, Hubert S, Lum J, Simoni Y, Malleret B, Zolezzi F, Chen J, Poidinger M, Satpathy AT, Briseno C, Wohn C, Malissen B, Murphy KM, Maini AA, Vanhoutte L, Guilliams M, Vial E, Hennequin L, Newell E, Ng LG, Musette P, Yona S, HaciniRachinel F, Ginhoux F. ,A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils., Immunity,2019 ,Mar 26. [Epub ahead of print].[Link]
11.Blériot C, Ng LG, Ginhoux F. ,"Cloaking" on Time: A Cover-Up Act by Resident Tissue Macrophages.,Cell.,2019, Apr 18;177(3):514-516..[Link]
12.Dress RJ, Dutertre CA, Giladi A, Schlitzer A, Low I, Shadan NB, Tay A, Lum J, Kairi MFBM, Hwang YY, Becht E, Cheng Y, Chevrier M, Larbi A, Newell EW, Amit I, Chen J, Ginhoux F. ,Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage.,Nat Immunol.,2019,Jul;20(7):852-864.[Link]
13.Dutertre CA, Becht E, Irac SE, Khalilnezhad A, Narang V, Khalilnezhad S, Ng PY, van den Hoogen LL, Leong JY, Lee B, Chevrier M, Zhang XM, Yong PJA, Koh G, Lum J, Howland SW, Mok E, Chen J, Larbi A, Tan HKK, Lim TKH, Karagianni P, Tzioufas AG, Malleret B, Brody J, Albani S, van Roon J, Radstake T, Newell EW, Ginhoux F. ,Single-Cell Analysis of Human Mononuclear Phagocytes Reveals SubsetDefining Markers and Identifies Circulating Inflammatory Dendritic Cells.,Immunity.,2019 ,Aug 27，[Epub ahead of print].[Link]
14.Bian Z, Gong Y, Huang T, Lee CZW, Bian L, Bai Z, Shi H, Zeng Y, Liu C, He J, Zhou J, Li X, Li Z, Ni Y, Ma C, Cui L, Zhang R, Chan JKY, Ng LG, Lan Y, Ginhoux F, Liu B. ,Deciphering human macrophage development at single-cell resolution.,Nature.,2020 ,Jun;582(7813):571-576..[Link]
15.Blériot C, Chakarov S, Ginhoux F.,Determinants of Resident Tissue Macrophage Identity and Function.,Jun 16;52(6):957-970.,2020, Immunity.[Link]
16.Silvin A, Chapuis N, Dunsmore G, Goubet AG, Dubuisson A, Derosa L, Almire C, Hénon C, Kosmider O, Droin N, Rameau P, Catelain C, Alfaro A, Dussiau C, Friedrich C, Sourdeau E, Marin N, Szwebel TA, Cantin D, Mouthon L, Borderie D, Deloger M, Bredel D, Mouraud S, Drubay D, Andrieu M, Lhonneur AS, Saada V, Stoclin A, Willekens C, Pommeret F, Griscelli F, Ng LG, Zhang Z, Bost P, Amit I, Barlesi F, Marabelle A, Pène F, Gachot B, André F, Zitvogel L, Ginhoux F, Fontenay M, Solary E.,Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19.,Cell,2020,Sep 17;182(6):1401-1418.e18..[Link]
Haiting Wang PhD student Email: haitingwang88@126.com Ziyi Li PhD Postdoctoral fellow Email: liziyie@pku.edu.cn Wei Guo PhD Postdoctoral fellow Email: guowei_med@shsmu.edu.cn Shuangyan Zhang Research Assistant Email: zsyan653@163.com Amanda Shin Research Assistant Email: amandashin@shsmu.edu.cn Zhaoyuan Liu PhD Assistant Professor Email: zhaoyuan_liu@sjtu.edu.cn