Shanghai Institute of Immunology & Department of Immunology and Microbiology
Shanghai Institute of Immunology & Department of Immunology and Microbiology

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Svetoslav Georgiev Chakarov

Email: svetoslav_chakarov@shsmu.edu.cn

Tel: 63846590-776796

Research Field: Myeloid cell biology and metabolism

Laboratory of Myeloid Cell Biology and Metabolism

Personal Introduction

Education：

  • 2009.9-2013.12 University Paul Sabatier (Toulouse, France), Immunology, Ph.D degree

  • 2007.9-2009.6 University Paul Sabatier (Toulouse, France), Immunology and Infectious Diseases, Master degree

  • 2004.9-2007.6 University Paul Sabatier (Toulouse, France), Cellular Biology and Physiology,  university degree

Work experience:

  • 2021.1-now Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai Institute of Immunology (SII), Research Fellow

  • 2019.2-2020.12 Singapore Immunology network; A*STAR, Research Scientist

  • 2014.2-2019.2 Singapore Immunology network; A*STAR, PostDoc

Research Interests:

Our Lab investigates how tissue environments shape immune cell identity and function, with a focus on myeloid cells. We combine single-cell and spatial multi-omics, advanced imaging, and genetic models to dissect how immune cells integrate niche-derived signals over time.

Our research aims to define fundamental principles governing tissue-resident immune cell specialization and to uncover how their dysregulation contributes to metabolic disease, fibrosis, and inflammation. We are particularly interested in immune–stromal interactions, stem cell niches, and the role of immune cells in tissue remodeling and homeostasis.

Research Interests include:

  • Immunometabolism and metabolic disease

  • Spatial and temporal regulation of immune cell identity

  • Stem cell niches and immune–stromal interactions

  • Single-cell and spatial omics

  • Single-cell and spatial omics

  • Tissue remodeling, fibrosis, and inflammation

Scientific Research Projects

  • Foreign Experts Project of Shanghai Science and Technology Commission, 2021.1-2021.12. Project No. 21WZ2503800

  • Start up from SJTU, 2021.1-2026.12

  • The National Natural Science Fund for Excellent Young Scientists Fund Program (Overseas), 2023.1-2025.12

  • The National Key R&D Program of China, 2024.1-2026.11, Project No. 2023YFC2306300

  • The general project of National Natural Science Foundation of China, 2025.1-2028.12, Project No. 32470920

  • National Natural Science Fund of China Research Fund for International Scientists, 2026.1-2027.12, Project No. W2532025

Publications

  1. Temporal and spatial atlas of eosinophil specialization across tissues. Hu Y*, Wu L, Qu S, Kong WT, Yu X, Xu J, Xu Z, He J, Wang C, Liu Z, Lu L, Ng LG, Blériot C, Su B, Ginhoux F, Li Z**, Chakarov S**. Nat Immunol. 2026 Feb;27(2):364-375. doi: 10.1038/s41590-025-02382-6.

  2. Septal LYVE1+ macrophages control adipocyte stem cell adipogenic potential. Yu X*, Hu Y*, Lim HY, Li Z, Jaitin DA, Yang K, Kong WT, Xu J, Bejarano DA, Bied M, Orliaguet L, Rengasamy G, Chow Z, Lee CZW, Lum J, Tian J, Zhang XM, Liu H, Tan SW, Chen J, See P, Loh YH, Malleret B, Baig S, Yassin MSM, Toh SES, Malissen B, Fu X, Kabashima K, Ng LG, Blériot C, Liu Z, Sheng L, Zheng DN, Qu J, Venteclef N, Su B, Amit I, Schlitzer A, Angeli V, Ginhoux F**, Chakarov S**. Science. 2025 Aug 28;389(6763):eadg1128. doi: 10.1126/science.adg1128.

  3. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Chakarov S*, Lim HY, Tan L, Lim SY, See P, Lum J, Zhang XM, Foo S, Nakamizo S, Duan K, Kong WT, Gentek R, Balachander A, Carbajo D, Bleriot C, Malleret B, Tam JKC, Baig S, Shabeer M, Toh SES, Schlitzer A, Larbi A, Marichal T, Malissen B, Chen J, Poidinger M, Kabashima K, Bajenoff M, Ng LG, Angeli V, Ginhoux F**. Science. 2019 Mar 15;363(6432). pii: eaau0964; doi: 10.1126/science.aau0964.

  4. Role of adipose tissue macrophages in obesity-related disorders. Chakarov S*, Blériot C, Ginhoux F**, J. Exp. Med. 2022 Jul 4;219(7):e20211948. doi: 10.1084/jem.20211948

  5. Cell-autonomous FLT3L shedding via ADAM10 mediates conventional dendritic cell development in mouse spleen. Fujita K*, Chakarov S*, Kobayashi T, Sakamoto K, Voisin B, Duan K, Nakagawa T, Horiuchi K, Amagai M, Ginhoux F, Nagao K** PNAS. 2019 Jul 16;116(29):14714-14723; doi: 10.1073/pnas.1818907116

  6. Determinants of resident tissue macrophage form and function. Bleriot C*, Chakarov S*, Ginhoux F**, Immunity 2020 Jun 16 52, 957-970; doi: 10.1016/j.immuni.2020.05.014

  7. Eosinophils in obesity and obesity-associated disorders. Hu Y* and Chakarov S**, Discovery Immunology 2023 Nov 14 Volume 2, Issue 1, 2023, doi: 10.1093/discim/kyad022

  8. Apolipoprotein E controls Dectin-1-dependent development of monocyte-derived alveolar macrophages upon pulmonary β-glucan-induced inflammatory adaptation. Theobald H, Bejarano DA, Katzmarski N, Haub J, Schulte-Schrepping J, Yu J, Bassler K, Ćirović B, Osei-Sarpong C, Piattini F, Vornholz L, Yu X, Sheoran S, Jawazneh A Al, Chakarov S, Haendler K, Brown GD, Williams DL, Bosurgi L, Ginhoux F, Ruland J, Beyer M, Greter M, Kopf M, Schultze JL, Schlitzer A.  Nat Immunol., 2024, 25:994-1006. doi: 10.1038/s41590-024-01830-z.

  9. Immunomodulatory Leptin Receptor+ Sympathetic Perineurial Cells Protect Against Obesity by Facilitating Neuroendocrine-Mediated Brown Adipose Tissue Thermogenesis. Haberman ER, Sarker G, Arús BA, Yilmaz-Özcan S, Martínez-Sánchez N, Freibergerová E, Fernández-González I, Zentai C, O’Brien CJO, Grainger E, Chakarov S, Raimondi A, Iannacone M, López M, Ginhoux F, Domingos AI, Immunity 2024 Jan 9;57(1):141-152.e5; doi: 10.1016/j.immuni.2023.11.006

  10. Dendritic cell type 3 arises from Ly6C+ monocyte-dendritic cell progenitors. Liu Z, Wang H, Li L, Dress RJ, Zhu Y, Zhang S, De Feo D, Kong WT, Cai P, Shin A, Piot C, Yu J, Gu Y, Zhang M, Gao C, Chen L, Wang H, Vétillard M, Guermonprez P, Kwok I, Ng LG, Chakarov S, Schlitzer A, Becher B, Dutertre CA, Su B, Ginhoux F, Immunity 2023 Aug 8;56(8):1761-1777.e6; doi: 10.1016/j.immuni.2023.07.001

  11. Dual ontogeny of disease-associated microglia and disease inflammatory macrophages in aging and neurodegeneration. Silvin A, Uderhardt S, Piot C, Da Mesquita S, Yang K, Geirsdottir L, Mulder K, Eyal D, Liu Z, Bridlance, C Thion MS, Zhang XM, Kong WT, Deloger M, Fontes V, Weiner A, Ee R, Dress R, Hang JW, Balachander A, Chakarov S, Malleret B, Dunsmore G, Cexus O, Chen J, Garel S, Dutertre CA, Amit I, Kipnis J, Ginhoux F, Immunity 2022 Aug 9;55(8):1448-1465.e6. doi: 10.1016/j.immuni.2022.07.004

  12. Unravelling the sex-specific diversity and functions of adrenal gland macrophages. Dolfi B, Gallerand A, Firulyova MM, Xu Y, Merlin J, Dumont A, Castiglione A, Vaillant N, Quemener S, Gerke H, Stunault MI, Schrank PR, Kim SH, Zhu A, Ding J, Gilleron J, Magnone V, Barbry P, Dombrowicz D, Duranton C, Wakkach A, Blin-Wakkach C, Becher B, Pagnotta S, Argüello RJ, Rantakari P, Chakarov S, Ginhoux F, Zaitsev K, Kim KW, Yvan-Charvet L, Guinamard RR, Williams JW, Ivanov S, Cell Rep. 2022 Jun 14;39(11):110949. doi: 10.1016/j.celrep.2022.110949

  13. ISM1 protects lung homeostasis via cell-surface GRP78-mediated alveolar macrophage apoptosis. Lam TYW, Nguyen N, Peh HY, Shanmugasundaram M, Chandna R, Tee JT, Ong CB, Hossain MZ, Venugopal S, Zhang T, Xu S, Tao Qiu, Kong WT, Chakarov S, Srivastava S, Liao W, Kim JS, The M, Ginhoux F, Wong FWS, Ge R, PNAS 2022 Jan 25;119(4):e2019161119; doi: 10.1073/pnas.2019161119

  14. A subset of Kupffer cells regulates metabolism through the expression of CD36. Bleriot C, Barreby E, Dunsmore G, Ballaire R, Chakarov S, Ficht X, De Simone G, Andreata F, Fumagalli V, Guo W, Wan G, Gessain G, Khalilnezhad A, Zhang XM, Ang N, Chen P, Morgantini C, Azzimato V, Kong WT, Liu Z, Pai R, Lum J, Shihui F, Low I, Xu C, Malleret B, Mohd Kairi MF, Balachander A, Cexus O, Larbi A, Lee B, Newell EW, Ng LG, Phoo WW, Sobota RM, Sharma A, Howland SW, Jinmiao Chen JM, Bajenoff M, Yvan-Charvet L, Venteclef N, Iannacone M, Aouadi M, and Ginhoux F, Immunity 2021 September 14 54, 1–16; doi: 10.1016/j.immuni.2021.08.006

  15. Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming. De Simone G, Andreata F, Bleriot C, Fumagalli V, Laura C, Garcia-Manteiga JM, Di Lucia P, Gilotto S,  Ficht X, De Ponti FF, Bono EB, Giustini L, Ambrosi G, Mainetti M, Zordan P, Benechet AP, Rava M, Chakarov S, Moalli F, Bajenoff M, Guidotti LG, Florent Ginhoux F and Iannacone M, Immunity 2021 September 14 54, 1–12; doi: 10.1016/j.immuni.2021.05.005

  16. Microbial exposure during early human development primes fetal immune cells. Mishra A, Lai GC, Yao LJ, Aung TT, Shental N, Rotter-Maskowitz A, Shepherdson E, Naranjan Singh G, Pai R, Shanti A, Men Men Wong R, Lee A, Khyriem C, Dutertre CA, Chakarov S, Srinivasan KG, Binte Shadan N, Zhang XM, Khalilnezhad S, Cottier F, Shin Min Tan A, Low G, Chen P, Fan Y, Xiang Hor P, Khoo May Lee A, Choolani M, Vermijlen D, Ankur Sharma A, Fuks G, Straussman R, Pavelka N, Malleret B, McGovern N, Albani S, Kok Yen Chan J, Ginhoux G, Cell 2021 June 24 184, 3394–3409; doi:10.1016/j.cell.2021.04.039

  17. Two populations of self-maintaining monocyte-independent macrophages exist in adult epididymis and testis. Wang M, Yang Y, Cansever D, Wang Y, Kantores C, Messiaen S, Moison D, Livera G, Chakarov S, Weinberger T, Stremmel C, Fijak M, Klein B, Pleuger C, Lian Z, Ma W, Liu O, Klee K, Händler K, Ulas T, Schlitzer A, Schultze JL, Becher B, Greter M, Liu Z, Ginhoux F, Epelman S, Schulz C, Meinhardt A, Bhushan S, PNAS 2021 Jan 5; 118(1) e2013686117; doi: 10.1073/pnas.2013686117

  18. Onco-fetal Reprogramming of Endothelial Cells Drives Immunosuppressive Macrophages in Hepatocellular Carcinom. Sharma A, Seow J, Dutertre CA, Pai R, Blériot C, Mishra A, Wong R, Singh G, Sudhagar S, Khalilnezhad S, Erdal S, Teo HM, Khalilnezhad A, Chakarov S, Kiat Hon Lim T, Chung Yaw Fui A, Kow Wei Chieh A, Cheow Peng Chung CP, Bonney G, Goh Kim Poh B, Chan J, Chow P, Ginhoux F, DasGupta R, Cell 2020 Oct 15 183, 1–18; doi: 10.1016/j.cell.2020.08.040

  19. Fate mapping via Ms4a3-expression history traces monocyte-derived cells. Liu Z, Gu Y, Chakarov S, Bleriot C, Kwok I, Chen X, Shin A, Huang W, Dress RJ, Dutertre CA, Schlitzer A, Chen J, Ng LG, Wang H, Liu Z, Su B, Ginhoux F. Cell. 2019 Sep 5 178, 1509-1525; doi:10.1016/j.cell.2019.08.009

  20. Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung. Schyns J, Bai O, Ruscitti C, Radermecker C, De Schepper S, Chakarov S, Farnir F, Pirottin D, Ginhoux F, Boeckxstaens G, Bureau F, Marichal T. Nat Commun. 2019 Sep 3; 10:3964; doi: 10.1038/s41467-019-11843-0

  21. Hyaluronan receptor lyve-1-expressing macrophages maintain arterial tone through hyaluronan-mediated regulation of smooth muscle cell collagen. Lim HY, Lim SY, Tan CK, Thiam CH, Goh CC, Carbajo D, Chew SHS, See P, Chakarov S, Wang XN, Lim LH, Johnson LA, Lum J, Fong CY, Bongso A, Biswas A, Goh C, Evrard M, Yeo KP, Basu R, Wang JK, Tan Y, Jain R, Tikoo S, Choong C, Weninger W, Poidinger M, Stanley ER, Collin M, Tan NS, Ng LG, Jackson DG, Ginhoux F, Angeli V Immunity. 2018 Aug 21;49(2):326-341.e7; doi: 10.1016/j.immuni.2018.06.008.

  22. Multiplex peptide-MHC tetramer staining using mass cytometry for deep analysis of the influenza-specific T-cell response in mice. Fehlings M, Chakarov S, Simoni Y, Sivasankar B, Ginhoux G, Newell EW Journal of Immunological Methods 2018 Feb 1; 453:30-36 doi: 10.1016/j.jim.2017.09.010

  23. Induced-pluripotent-stem-cell-derived primitive macrophages provide a platform for modeling tissue-resident macrophage differentiation and function. Takata K, Kozaki T, Lee CZW, Thion MS, Otsuka M, Lim S, Utami KH, Fidan K, Park DS, Malleret B, Chakarov S, See P, Low D, Low G, Garcia-Miralles M, Zeng R, Zhang J, Goh CC, Gul A, Hubert S, Lee B, Chen J, Low I, Shadan NB, Lum J, Wei TS, Mok E, Kawanishi S, Kitamura Y, Larbi A, Poidinger M, Renia L, Ng LG, Wolf Y, Jung S, Önder T, Newell E, Huber T, Ashihara E, Garel S, Pouladi MA, Ginhoux F. Immunity. 2017 Jul 18;47(1):183-198.e6; doi: 10.1016/j.immuni.2017.06.017.

  24. Exposure to bacterial CpG DNA protects from airway allergic inflammation by expanding regulatory lung interstitial macrophages. Sabatel C, Radermecker C, Fievez L, Paulissen G, Chakarov S, Fernandes C, Olivier S, Toussaint M, Pirottin D, Xiao X, Quatresooz P, Sirard JC, Cataldo D, Gillet L, Bouabe H, Desmet CJ, Ginhoux F, Marichal T, Bureau F Immunity. 2017 Mar 21;46(3):457-473; doi: 10.1016/j.immuni.2017.02.016.

  25. Unsupervised high-dimensional analysis aligns dendritic cells across tissues and species. Guilliams M, Dutertre CA, Scott CL, McGovern N, Sichien D, Chakarov S, Van Gassen S, Chen J, Poidinger M, De Prijck S, Tavernier SJ, Low I, Irac SE, Mattar CN, Sumatoh HR, Low GHL, Chung TJK, Chan DKH, Tan KK, Hon TLK, Fossm E, Bogen B, Choolani M, Chan JKY, Larbi A, Luche H, Henri S, Saeys Y, Newell EW, Lambrecht BN, Malissen B, Ginhoux F. Immunity. 2016 Sep 20;45(3):669-684; doi:10.1016/j.immuni.2016.08.015. Epub 2016 Sep13.

  26. Mpath maps multi-branching single-cell trajectories revealing progenitor cell progression during development. Chen J, Schlitzer A, Chakarov S, Ginhoux F, Poidinger M Nat Commun. 2016 Jun 30; 7:11988; doi:10.1038/ncomms11988.

  27. Expansion and activation of CD103(+) dendritic cell progenitors at the tumor site enhances tumor responses to therapeutic PD-L1 and BRAF inhibition. Salmon H, Idoyaga J, Rahman A, Leboeuf M, Remark R, Jordan S, Casanova-Acebes M, Khudoynazarova M, Agudo J, Tung N, Chakarov S, Rivera C, Hogstad B, Bosenberg M, Hashimoto D, Gnjatic S, Bhardwaj N, Palucka AK, Brown BD, Brody J, Ginhoux F, Merad M. Immunity. 2016 Apr 19;44(4):924-38; doi:10.1016/j.immuni.2016.03.012.

  28. Tracking by flow cytometry antigen-specific follicular helper T cells in wild-type animals after protein vaccination. Chakarov S* & Fazilleau N** Methods Mol Biol. 2015 1291:39-47; doi: 10.1007/978-1-4939-2498-1_4.

  29. Monocyte-derived dendritic cells promote T follicular helper cell differentiation. Chakarov S* & Fazilleau N** EMBO Mol Med. 2014 May;6(5):590-603; doi: 10.1002/emmm.201403841.

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