Educational Background

• 2017-2021 ShanghaiTech University, MA.Sc in Biochemistry and molecular biology

• 2013-2017 South China Agriculture University, B.Sc in Biology Science

Professional Experience

• 2021-present Technician in Center for Single-Cell Omics, Shanghai Jiao Tong University

Research Interests

• Technology development based on laser capture microdissection - magnetic trace analysis associated with proteomics and to better achieve the spatial resolution of proteomics;

• To construct the untargeted metabolomics experimental platform and provide technical services related to proteomics and metabolomics.

Research Experience

1. Extraction of plasma low molecular weight protein and peptide (LMWP):

• Evaluation of extraction LMWP methods;

• Routine proteomics workflow, such as multiple digestion methods, liquid chromatography separation technology and mass spectrometry acquisition methods and settings;

• Comparison of database searching software and parameters.

2. Practical experience about validation of biomarker associated with proteomics:

• Immunohistochemistry and other related tissue microarray techniques;

• Information mining and data analysis from database (e.g., TCGA);

• Quantification of specific peptides using selected reaction monitoring (SRM) / parallel reaction monitoring (PRM) based on mass spectrometry;

• Co-immunoprecipitation operation;

• Western blot operation.

3. Composition analysis of high-density lipoprotein associated with coronary artery disease based on proteomics and lipidomics:

• To isolate high density lipoprotein (HDL) using a new method - weak anion exchange chromatography;

• To acquire proteome and lipidome of HDL;

• To draw protein and lipid molecular characteristics of coronary artery disease.

4. Glycosylation sites modification analysis of apolipoprotein associated with coronary artery disease

• Apolipoprotein enrichment and its proteome preparation and optimization;

• To reveal the main type of glycosylation modification in patients with coronary heart disease and to find association of glycosylation modification with disease progression.

5. To characterize features of hepatocellular carcinoma through multi-omics data analysis, including proteomics, phosphoproteomics and transcriptomics.

Publications

1. Li C#, Sun YD#, Yu GY#, Cui JR#, Lou Z#, Zhang H, Huang Y, Bai CG, Deng LL, Liu P, Zheng K, Wang YH, Wang QQ, Li QR, Wu QQ, Liu Q, Shyr Y, Li YX, Chen LN, Wu JR, Zhang W, Zeng R*. Integrated Omics of Metastatic Colorectal Cancer. Cancer Cell. 2020 Nov 9;38(5):734-747.e9.

2. Peng GX#, Zhang Y, Wang QQ, Li QR, Xu H, Wang ED*, Zhou XL*. The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. Nucleic Acids Res. 2021 Mar 18;49(5):2816-2834.

3. Li CY#, Han TT#, Li QR#, Zhang MH#, Guo R, Yang Y, Lu WL, Li ZW, Peng C, Wu P, Tian XX, Wang QQ, Wang YX, Zhou V, Han ZY, Li HC*, Wang F*, Hu RG*. MKRN3-mediated ubiquitination of Poly(A)-binding proteins modulates the stability and translation of GNRH1 mRNA in mammalian puberty. Nucleic Acids Res. 2021 Mar 21:gkab155.